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smDCP——用于双带选择性异核传递的魔角旋转固体NMR方法

(J. Magn. Reson., 2012, 217, 92-99) 

  本文描述了一种魔角旋转条件下用于稀核间磁化传递的DCP方法。在15N-13C CP期间,13C射频场采用Sine调制,15N射频场为Tangent调制。Sine调制使13C射频场的在发射机中心两端的两条频率带上产生有效的锁场,从而实现15N磁化同时传向两条频率带上的13C自旋。实验和模拟显示:Sine调制选择性地使15N磁化同时传向与之键连的残基内13和残基间13C’羰基),从而在一个实验内同时建立蛋白质内残基内和残基间15N-13C相关,有利于蛋白质的信号归属。该方法的选择性和有效性利用了U-13C,15N标记GB1微晶蛋白证实。 

  

Dual-band selective double cross polarization for heteronuclear polarization transfer between dilute spins in solid-state MAS NMR 

Abstract    A sinusoidal modulation scheme is described for selective heteronuclear polarization transfer between two dilute spins in double cross polarization magic-angle-spinning nuclear magnetic resonance spectroscopy. During the second N → C cross polarization, the 13C RF amplitude is modulated sinusoidally while the 15N RF amplitude is tangent. This modulation induces an effective spin-lock field in two selective frequency bands in either side of the 13C RF carrier frequency, allowing for simultaneous polarization transfers from15N to 13C in those two selective frequency bands. It is shown by experiments and simulations that this sinusoidal modulation allows one to selectively polarize from 15N to its covalently bonded 13Cα and 13C’ carbons in neighboring peptide planes simultaneously, which is useful for establishing the backbone connectivity between two sequential residues in protein structural elucidation. The selectivity and efficiency were experimentally demonstrated on a uniformly 13C,15N-labeled β1 immunoglobulin binding domain of protein G (GB1). 

 








 
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